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Virulence gene distribution in clinical, nosocomial and environmental isolates of Pseudomonas aeruginosa

journal contribution
posted on 2017-12-06, 00:00 authored by Richard Bradbury, L Roddam, A Merritt, D Reid, A Champion
The virulence factor genotypes of a large cohort of clinical, nosocomial environment and community environment isolates (184 in total) of Pseudomonas aeruginosa from Tasmania, Australia, were determined by PCR. The virulence factor genotype of the majority of isolates was highly conserved, with the exception of the virulence gene exoU, which demonstrated low prevalence (33 isolates; 18 %) in the population tested. Isolates collected from the environment of intensive therapy wards (intensive care unit and neurosurgical units) of the major tertiary referral hospital in Tasmania were found to be more likely (P<0.001 and P<0.05, respectively) to possess the virulence factor gene exoU than all other isolates. Adult cystic fibrosis isolates showed a decreased prevalence of the exoU gene (P<0.01) when compared to other clinical isolates (P<0.01), which may indicate decreased virulence. No specific virulence factor genotype was associated with the cystic fibrosis epidemic strains tested.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

59

Issue

8

eISSN

1473-5644

ISSN

0022-2615

Location

UK

Publisher

Society for General Microbiology

Language

en-aus

Peer Reviewed

  • Yes

Open Access

  • No

External Author Affiliations

PathWest Laboratory Medicine; Royal Hobart Hospital; TBA Research Institute; University of Tasmania;

Era Eligible

  • Yes

Journal

Journal of medical microbiology.

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