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Trehalose promotes the survival of Saccharomyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress

journal contribution
posted on 06.12.2017, 00:00 authored by A Bandara, S Fraser, P Chambers, Grant StanleyGrant Stanley
Trehalose is known to protect cells from various environmental assaults; however, its role in the ethanol tolerance of Saccharomyces cerevisiae remains controversial. Many previous studies report correlations between trehalose levels and ethanol tolerance across a variety of strains, yet variations in genetic background make it difficult to separate the impact of trehalose from other stress response factors. In the current study, investigations were conducted on the ethanol tolerance of S. cerevisiae BY4742 and BY4742 deletion strains, tsl1D and nth1D, across a range of ethanol concentrations. It was found that trehalose does play a role in ethanol tolerance at lethal ethanol concentrations, but not at sublethal ethanol concentrations; differences of 20–40% in the intracellular trehalose concentration did not provide any growth advantage for cells incubated in the presence of sublethal ethanol concentrations. It was speculated that the ethanol concentration-dependent nature of the trehalose effect supports a mechanism for trehalose in protecting cellular proteins from the damaging effects of ethanol.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

9

Start Page

1208

End Page

1216

Number of Pages

9

eISSN

1567-1364

ISSN

1567-1356

Location

UK

Publisher

Wiley-Blackwell

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Australian Wine Research Institute; Victoria University (Melbourne, Vic.);

Era Eligible

Yes

Journal

FEMS yeast research.