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Translocation and dissemination of commensal bacteria in post-stroke infection
journal contribution
posted on 2018-08-31, 00:00 authored by Dragana StanleyDragana Stanley, LJ Mason, KE Mackin, YN Srikhanta, D Lyras, MD Prakash, K Nurgali, A Venegas, MD Hill, RJ MooreBacterial infection is highly prevalent in patients who have had a stroke. Despite the potential contribution of micro-aspiration in post-stroke pneumonia, we found that the majority of the microorganisms detected in the patients who developed infections after having a stroke were common commensal bacteria that normally reside in the intestinal tracts. In a mouse model of ischemic stroke, post-stroke infection was only observed in mice that were born and raised in specific-pathogen-free facilities; this was not seen in mice that were born and raised in germ-free facilities. Using high-throughput 16S rRNA gene amplicon sequencing and bioinformatics analyses, we provide evidence demonstrating that the source of the bacteria forming the microbial community in the lungs of post-stroke mice was indeed the host small intestine. Additionally, stroke-induced gut barrier permeability and dysfunction preceded the dissemination of orally inoculated bacteria to peripheral tissues. This study identifies a novel pathway in which stroke promotes the translocation and dissemination of selective strains of bacteria that originated from the host gut microbiota.
Funding
Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)
History
Volume
22Issue
11Start Page
1277End Page
1284Number of Pages
8eISSN
1546-170XISSN
1078-8956Location
United StatesPublisher
Nature Publishing GroupPublisher DOI
Full Text URL
Language
engPeer Reviewed
- Yes
Open Access
- No
External Author Affiliations
Monash University; Victoria University; University of Calgary, Canada;Era Eligible
- Yes