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Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes

journal contribution
posted on 13.02.2019, 00:00 by M Khorramdelazad, I Bar, P Whatmore, G Smetham, V Bhaaskaria, Y Yang, Shahla Hosseini Bai, N Mantri, Y Zhou, R Ford
Background: Ascochyta blight, caused by the fungus Ascochyta lentis, is one of the most destructive lentil diseases worldwide, resulting in over $16 million AUD annual loss in Australia alone. The use of resistant cultivars is currently considered the most effective and environmentally sustainable strategy to control this disease. However, little is known about the genes and molecular mechanisms underlying lentil resistance against A. lentis. Results: To uncover the genetic basis of lentil resistance to A. lentis, differentially expressed genes were profiled in lentil plants during the early stages of A. lentis infection. The resistant 'ILL7537' and susceptible 'ILL6002' lentil genotypes were examined at 2, 6, and 24 h post inoculation utilising high throughput RNA-Sequencing. Genotype and time-dependent differential expression analysis identified genes which play key roles in several functions of the defence response: fungal elicitors recognition and early signalling; structural response; biochemical response; transcription regulators; hypersensitive reaction and cell death; and systemic acquired resistance. Overall, the resistant genotype displayed an earlier and faster detection and signalling response to the A. lentis infection and demonstrated higher expression levels of structural defence-related genes. Conclusions: This study presents a first-time defence-related transcriptome of lentil to A. lentis, including a comprehensive characterisation of the molecular mechanism through which defence against A. lentis is induced in the resistant lentil genotype. © 2018 The Author(s).

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

19

Start Page

1

End Page

21

Number of Pages

21

eISSN

1471-2164

Publisher

BioMed Central, UK

Additional Rights

CC BY 4.0

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

17/01/2018

External Author Affiliations

RMIT University; University of Melbourne; Griffith University; University of the Sunshine Coast; e National Institute of Nutrition and Seafood Research (NIFES), Norway

Era Eligible

Yes

Journal

BMC Genomics