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Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix

journal contribution
posted on 28.10.2020, 00:00 by SS Wanyonyi, A Kumar, Ryan du Preez, C Lefevre, KR Nicholas
Background The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation. Methods WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq. Results ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210). Conclusions A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified. General significance This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function. © 2017 The Authors

Funding

Category 3 - Industry and Other Research Income

History

Volume

12

Start Page

120

End Page

128

Number of Pages

9

eISSN

2405-5808

Publisher

Elsevier

Additional Rights

CC BY-NC-ND 4.0

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

28/08/2017

External Author Affiliations

Deakin University; University of Southern Queensland

Era Eligible

Yes

Journal

Biochemistry and Biophysics Reports

Exports

CQUniversity

Exports