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Transcriptional changes associated with ethanol tolerance in saccharomyces cerevisiae

journal contribution
posted on 06.12.2017, 00:00 by P Chambers, Grant StanleyGrant Stanley, A Borneman, S Fraser, Dragana StanleyDragana Stanley
Saccharomyces spp. are widely used for ethanol production; however, fermentation productivity is negatively affected by the impact of ethanol accumulation on yeast metabolic rate and viability. This study used microarray and statistical two-way ANOVA analysis to compare and evaluate gene expression profiles of two previously generated ethanol-tolerant mutants, CM1 and SM1, with their parent, Saccharomyces cerevisiae W303-1A, in the presence and absence of ethanol stress. Although sharing the same parentage, the mutants were created differently: SM1 by adaptive evolution involving long-term exposure to ethanol stress and CM1 using chemical mutagenesis followed by adaptive evolution-based screening. Compared to the parent, differences in the expression levels of genes associated with a number of gene ontology categories in the mutants suggest that their improved ethanol stress response isa consequence of increased mitochondrial and NADH oxidation activities, stimulating glycolysis and other energy yielding pathways. This leads to increased activity of energy demanding processes associated with the production of proteins and plasma membrane components, which are necessary for acclimation to ethanol stress. It is suggested that a key function of the ethanol stress response is restoration of the NAD+/NADH redox balance, which increasingly ceraldehyde-3-phosphate dehydrogenase activity, and higher glycolytic flux in the ethanol-stressed cell. Both mutants achieved this by a constitutive increase in carbon flux in the glycerol pathway as a means of increasing NADH oxidation.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

88

Start Page

231

End Page

239

Number of Pages

9

eISSN

1432-0614

ISSN

0175-7598

Location

Berlin, Germany

Publisher

Springer Verlag

Language

en-aus

Peer Reviewed

Yes

Open Access

No

Era Eligible

Yes

Journal

Applied microbiology and biotechnology.