cqu_14930+bine8e3a3ec-09b8-44c0-bd9f-168540b7b676+bine8e3a3ec-09b8-44c0-bd9f-168540b7b676.0.pdf (1.16 MB)
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The time-course of broiler intestinal microbiota development after administration of cecal contents to incubating eggs

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journal contribution
posted on 23.05.2018, 00:00 by Emma DonaldsonEmma Donaldson, Dragana StanleyDragana Stanley, RJ Hughes, RJ Moore
Background. The microbial populations that inhabit the gastrointestinal tract (GIT) are known to influence the health and growth performance of the host. Clean hatcheries and machine-based incubation practices in the commercial poultry industry can lead to the acquisition of aberrant microbiota in the GIT of chickens and a very high level of bird-to-bird variation. The lack of microbial profile flock uniformity presents challenges for harnessing and manipulating intestinal bacteria to better serve the host. Methods. Cecal contents from high or low performing chickens were used to inoculate the surface of eggs prior to hatching and then the initial gut colonisation was monitored and subsequent changes in gut microbiota composition were followed over time. Two different cecal treatment groups were compared to an untreated control group (n=32). Bacterial communities were characterised using high-throughput 16S rRNA gene sequencing techniques. Results. Cecal microbiota transfer via egg surface application did not transfer the performance profile of the donors to the recipient birds. One of the cecal inoculations provided a more uniform gut microbiota, but this was not reproduced in the second group with a different inoculum. Development of the intestinal community was reproducible in all three groups with some genera like Lactobacillus showing no change, others like Faecalibacterium increased in abundance slowly and steadily over time and others like Enterobacter were abundant only in the first days of life. Discussion. The cecal treatment reduced bird-to-bird variation in microbiota compo-sition. Although the high FCR performance of donor birds was not transferred with the cecal microbiota, all three groups, including the control, performed better than standard for the breed. The pattern of microbiota development was similar in all three flocks, indicating that the normal processes of microbiota acquisition largely swamped any effect of the cecal material applied to eggs. © 2017 Donaldson et al.

Funding

Category 4 - CRC Research Income

History

Volume

5

Start Page

1

End Page

19

Number of Pages

19

eISSN

2167-8359

Publisher

PeerJ, UK

Additional Rights

CC-BY 4.0

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

27/06/2017

External Author Affiliations

University of New England; South Australian Research and Development Institute; The University of Adelaide; RMIT

Author Research Institute

Institute for Future Farming Systems

Era Eligible

Yes

Journal

PeerJ

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