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The plasma membrane of microaerophilic protists : oxidative and nitrosative stress

journal contribution
posted on 06.12.2017, 00:00 by D Lloyd, J Harris, G Biagini, M Hughes, S Maroulis, C Bernard, R Wadley, M Edwards
The trans-plasma-membrane electrochemical potential of microaerophilic protists was monitored by the use of voltage-sensitive charged lipophilic fluorophores; of the many availableprobes, the anionic oxonol dye bis (1,3-dibarbituric acid)-trimethine oxonol [DiBAC4(3)] is an example of one which has been successfully employed using fluorescence microscopy, confocal laser-scanning microscopy and flow cytometry. Several microaerophilic protists have been investigated with this dye; these were Giardia intestinalis, Trichomonas vaginalis, Tritrichomonas foetus, Hexamita inflata and Mastigamoeba punctachora. Under conditions where they exhibit normal vitality, these organisms exclude DiBAC4(3) by virtue of their maintenance of a plasma-membrane potential (negative inside). Uptake of the fluorophore is indicative of disturbance to this membrane (i.e. by inhibition of pump/leak balance, blockage of channels or generation of ionic leaks), and is indicative of metabolic perturbation or environmental stress. Here, it is shown that oxidative or nitrosative stress depolarizes the plasma membranes of the aforementioned O2-sensitive organisms and allows DiBAC4(3) influx. Oxonol uptake thereby provides a sensitive and early indication of plasma-membrane perturbation by agents that may lead to cytotoxicity and eventually to cell death by necrotic or apoptotic pathways.

History

Volume

150

Start Page

1183

End Page

1190

Number of Pages

8

eISSN

1465-2080

ISSN

1350-0872

Location

United Kingdom

Publisher

Society for General Microbiology

Language

en-aus

Peer Reviewed

Yes

Open Access

No

Era Eligible

Yes

Journal

Microbiology.

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