This study examined the effect of diamond deloading tape on medial gastrocnemius (MG) muscle behaviour during exercise in healthy adults (n = 27). A randomised cross-over trial assessed the effect of tape (no-tape, sham-tape and deload-tape) on ankle and MG fascicle kinematics during three heel raise-lower exercises [double leg (DL), single leg (SL) and loaded single leg (LSL)]. There was no effect of tape on standing fascicle length (FL) or pennation angle (PA), or ankle or knee joint angle. There was a significant effect of tape on ankle kinematics for all exercises. Both the deload-tape and sham-tape resulted in less ankle plantar flexion but had no effect on dorsiflexion. There was a significant effect of tape on FL change for the SL and LSL exercise. Compared to no-tape, the deload-tape resulted in less fascicle shortening during ankle plantar flexion, and more fascicle lengthening during ankle dorsiflexion. For the LSL exercise, deload-tape caused MG fascicles to operate at longer lengths, for a given joint angle. Diamond taping, with or without added tension, has only a small effect on ankle and MG fascicle kinematics during the heel raise-lower exercise. With the exception of the LSL exercise, both tape conditions resulted in similar changes in the FL-angle relations.