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The Well-of-the-Well system : an efficient approach to improve embryo development

journal contribution
posted on 06.12.2017, 00:00 authored by Gabor VajtaGabor Vajta, T Korösi, Y Du, K Nakata, S Ieda, M Kuwayama, Z Nagy
Transfer of human embryos at the blastocyst stage may offer considerable benefits including an increased implantation rate and a decreased risk of multiple pregnancies; however, blastocyst culture requires an efficient and reliable in-vitro embryo culture system. In this study, the effect of the Well-of-the-Well (WOW) system consisting of microwells formed on the bottom of the culture dish was tested in three mammalian species, including humans. The WOW system resulted in significant improvement when comparing the drops for culture of in-vitro-matured and parthenogenetically activated porcine oocytes, and in-vivo-derived mouse zygotes. In human embryos, using a sibling oocyte design, embryos cultured in WOW developed to the blastocyst stage in a significantly higher proportion than did embryos cultured traditionally (55% in WOW and 37% in conventional culture; P < 0.05). In a separate study, also in human, a total of 48 patients with a cumulative 214 unsuccessful previous IVF cycles were selected for the trials. In subsequent intracytoplasmic sperm injection cycles, oocytes/embryos were cultured individually in the WOW system or in microdrops. Transferable quality blastocyst development (48.9% of cultured zygotes) was observed in the WOW system. Ninety-four blastocysts transferred to 45 patients resulted in clinical pregnancy rates of 48.9%, including nine twin pregnancies, seven single pregnancies, five miscarriages and one ectopic pregnancy. The results indicate that the WOW system provides a promising alternative for microdrop culture of mammalian embryos, including human embryos.

History

Volume

17

Issue

1

Start Page

73

End Page

81

Number of Pages

9

eISSN

1472-6491

ISSN

1472-6483

Publisher

Elsevier Ltd

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Beijing ji yin zu yan jiu suo; Kato Ladies’ Clinic, Shinjuku; Kaáli Intézet; PIVET Medical Centre, Perth; Reproductive Biology Associates (Atlanta); TBA Research Institute;

Era Eligible

Yes

Journal

Reproductive biomedicine online.