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Spectrophotometer ageing and prediction of fruit attributes

Deterioration of lamp output quality over time and degradation of detector signal to noise ratio are issues associated with ageing of a spectrophotometer. To document the effect of instrument ageing on short wavelength near infrared spectroscopy-based assessment of internal attributes of fruit quality prediction (total soluble solids, TSS, of juice), an assessment was conducted of several handheld PDA based spectrophotometers over several years, with repeated spectra of a reference Teflon (PFTE) tile and spectra of 20 apple fruit acquired at yearly intervals. The repeatability of each instrument was assessed as the standard deviation of absorbance of repeated measures of a reference, typically around 0.2mAbs. Instrument changes were identified in performance and in principal components analysis plots, but performance (apple TSS model) was not related to instrument repeatability. A piecewise direct standardisation method is recommended to maintain a multivariate calibration model across spectrometers.

Funding

Category 3 - Industry and Other Research Income

History

Volume

24

Issue

4

Start Page

337

End Page

344

Number of Pages

8

eISSN

1751-6552

ISSN

0967-0335

Publisher

Journal of Near Infrared Spectroscopy

Peer Reviewed

Yes

Open Access

No

Era Eligible

Yes

Journal

Journal of Near Infrared Spectroscopy