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Species-specific PCR primers for the mitochondrial genome control region hypervariable region 1 of the reef fish Lutjanus sebae

journal contribution
posted on 06.12.2017, 00:00 by William Aspden, Graham Pegg, Leica Briskey, W Sinclair
Lutjanus sebae is an important commercial and recreational fish found throughout the Indo-West Pacific region. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1) we isolated a 385 bp fragment of HVR1 then designed specific primers near each end of this sequence in the conserved regions flanking the hypervariable region. These primers were shown to yield good quantity and quality of product and to be species specific. Of 20 L sebae studied there were 8 segregating sites and 8 haplotypes. These primers will provide a useful tool for population genetics studies, identification of larvae and eggs and for wildlife forensics.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

6

Issue

2

Start Page

499

End Page

509

Number of Pages

11

ISSN

1471-8278

Location

Malden, USA

Publisher

Blackwell Publishing

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Faculty of Sciences, Engineering and Health; Marine Molecular Genetics and Biotechnology Group; TBA Research Institute;

Era Eligible

Yes

Journal

Molecular ecology notes.

Exports