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Seminal plasma proteome of electroejaculated Bos indicus bulls

journal contribution
posted on 14.08.2018, 00:00 by JPA Rego, JM Crisp, AA Moura, AS Nouwens, Y Li, B Venus, Nicholas CorbetNicholas Corbet, DH Corbet, BM Burns, GB Boe-Hansen
The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-month old Australian Brahman sires were evaluated and subjected to electroejaculation. Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass spectrometry. The percentage of progressively motile and morphologically normal sperm of the bulls were 70.4. ±. 2.3 and 64. ±. 3.2%, respectively. A total of 108 spots were identified in the 2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all spots detected in the maps and spermadhesins comprised the second most abundant constituents. Other proteins of the Bos indicus seminal plasma include clusterin, albumin, transferrin, metalloproteinase inhibitor 2, osteopontin, epididymal secretory protein E1, apolipoprotein A-1, heat shock 70. kDa protein, glutathione peroxidase 3, cathelicidins, alpha-enolase, tripeptidyl-peptidase 1, zinc-alpha-2-glycoprotein, plasma serine protease inhibitor, beta 2-microglobulin, proteasome subunit beta type-4, actin, cathepsins, nucleobinding-1, protein S100-A9, hemoglobin subunit alpha, cadherin-1, angiogenin-1, fibrinogen alpha and beta chain, ephirin-A1, protein DJ-1, serpin A3-7, alpha-2-macroglobulin, annexin A1, complement factor B, polymeric immunoglobulin receptor, seminal ribonuclease, ribonuclease-4, prostaglandin-H2 d-isomerase, platelet-activating factor acetylhydrolase, and phosphoglycerate kinase 1. In conclusion, this work uniquely portrays the Bos indicus seminal fluid proteome, based on samples from a large set of animals representing the Brahman cattle of the tropical Northern Australia. Based on putative biochemical attributes, seminal proteins act during sperm maturation, protection, capacitation and fertilization. © 2014 .

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

148

Issue

1-2

Start Page

1

End Page

17

Number of Pages

17

ISSN

0378-4320

Publisher

Elsevier BV

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

The Federal University of Ceará, Brazil; University of Queensland; CSIRO; Department of Agriculture, Fisheries and Forestry; Queensland Alliance for Agriculture and Food Innovation

Era Eligible

Yes

Journal

Animal Reproduction Science