File(s) not publicly available
Retinal explant culture: A platform to investigate human neuro-retina
journal contribution
posted on 2019-07-17, 00:00 authored by A Murali, Charmaine Ramlogan-SteelCharmaine Ramlogan-Steel, S Andrzejewski, Jason SteelJason Steel, CJ LaytonThe retina is the tissue responsible for light detection, in which retinal neurons convert light energy into electrical signals to be transported towards the visual cortex. Damage of retinal neurons leads to neuronal cell death and retinal pathologies, compromising visual acuity and eventually leading to irreversible blindness. Models of retinal neurodegeneration include 2D systems like cell lines, disassociated cultures and co-cultures, and 3D models like organoids, organotypic retinal cultures and animal models. Of these, ex-vivo human retinal cultures are arguably the most suitable models for translational research as they retain complex inter-cellular interactions of the retina and precisely mimic in-situ responses. In this review, we summarise the distinguishing features of the human retina which are important to preserve in experimental culture, the historical development of human retinal culture systems, the factors affecting ex-vivo human retinal culture and the applications and challenges associated with current methods of human retinal explant culture.
History
Volume
47Issue
2Start Page
274End Page
285Number of Pages
12eISSN
1442-9071ISSN
1442-6404Publisher
Wiley-Blackwell Publishing Asia, AustraliaPublisher DOI
Peer Reviewed
- Yes
Open Access
- No
Acceptance Date
2018-10-22External Author Affiliations
Translational Research Institute, QLD; University of QueenslandEra Eligible
- Yes