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Retinal explant culture: A platform to investigate human neuro-retina

journal contribution
posted on 17.07.2019, 00:00 by A Murali, Charmaine Ramlogan-SteelCharmaine Ramlogan-Steel, S Andrzejewski, Jason SteelJason Steel, CJ Layton
The retina is the tissue responsible for light detection, in which retinal neurons convert light energy into electrical signals to be transported towards the visual cortex. Damage of retinal neurons leads to neuronal cell death and retinal pathologies, compromising visual acuity and eventually leading to irreversible blindness. Models of retinal neurodegeneration include 2D systems like cell lines, disassociated cultures and co-cultures, and 3D models like organoids, organotypic retinal cultures and animal models. Of these, ex-vivo human retinal cultures are arguably the most suitable models for translational research as they retain complex inter-cellular interactions of the retina and precisely mimic in-situ responses. In this review, we summarise the distinguishing features of the human retina which are important to preserve in experimental culture, the historical development of human retinal culture systems, the factors affecting ex-vivo human retinal culture and the applications and challenges associated with current methods of human retinal explant culture.

History

Volume

47

Issue

2

Start Page

274

End Page

285

Number of Pages

12

eISSN

1442-9071

ISSN

1442-6404

Publisher

Wiley-Blackwell Publishing Asia, Australia

Peer Reviewed

Yes

Open Access

No

Acceptance Date

22/10/2018

External Author Affiliations

Translational Research Institute, QLD; University of Queensland

Era Eligible

Yes

Journal

Clinical and Experimental Ophthalmology

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