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Regulatory T lymphocytes are associated with increased nasopharyngeal colonization in children

journal contribution
posted on 25.03.2019, 00:00 by Jessica BrowneJessica Browne, EH Matthews, Andrew Taylor-RobinsonAndrew Taylor-Robinson, JM Kyd
Objectives: Regulatory T lymphocytes (Treg) have been linked to survival of commensal bacteria at mucosal sites, but their presence and role in chronic otitis media (COM) and their response to otopathogens has not been evaluated previously. We investigated the association between Treg lymphocytes and otopathogens in COM prone and non-COM prone children. Methods: Forty children, 2 to 7 years of age, scheduled for adenoidectomy were enrolled into COM (n = 20) or non-COM (n = 20) groups. Adenoid biopsy and nasopharyngeal aspirate bacteriology were assessed by conventional culture techniques. Peripheral blood and adenoid lymphocytes were stained with viability stain, monoclonal anti-CD19, anti-CD3, anti-CD4, anti-CD8, anti-CD25 and anti-CD127. Cells were stained intracellularly with monoclonal anti-FoxP3 and then quantified by flow cytometry. Results: Children with nasopharyngeal otopathogen-positive culture had significantly more circulating CD3+CD4+FoxP3+CD25hi+CD127lo+ lymphocytes (M = 4.4%) compared to culture-negative children (M = 3.1%, p = 0.005). Circulating CD19+ lymphocytes were significantly increased in children with positive Moraxella catarrhalis nasopharyngeal culture (M = 12.4%) compared to culture-negative children (M = 8.6%, p = 0.006). Adenoid-derived lymphocytes were not significantly different in children with any positive nasopharyngeal culture compared to negative culture. Lymphocyte subsets were not significantly different between COM and non-COM prone children. Conclusion: Clinically-detectable otopathogen nasopharyngeal culture is positively associated with Treg lymphocytes, potentially inducing suppressive effector responses to promote colonization and infection chronicity. This finding supports further investigation of Treg lymphocyte activity and influence on upper airway colonization of young children.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

120

Start Page

51

End Page

57

Number of Pages

7

eISSN

1872-8464

ISSN

0165-5876

Publisher

Elsevier BV, UK

Language

en

Peer Reviewed

Yes

Open Access

No

Acceptance Date

03/02/2019

External Author Affiliations

Mater Misericordiae Rockhampton Hospital, Australia; Swinburne University of Technology, Melbourne, Australia

Era Eligible

Yes

Journal

International Journal of Pediatric Otorhinolaryngology