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Rapid detection of Clostridium difficile toxins from stool samples using real-time multiplex PCR

journal contribution
posted on 2024-06-17, 01:52 authored by A Pallis, Jalal Jazayeri, P Ward, K Dimovski, S Svobodova
In this study, a total of 650 stool samples were tested to show that our method is capable of detecting four Clostridium difficile genes; tcdA, tcdB, encoding toxin A (TcdA) and toxin B (TcdB), and the binary toxin C. difficile transferase genes (cdtA and/or cdtB) encoding CDT toxin. Besides detecting the targeted C. difficile genes, our method can be used to detect the presence of any inhibitory components in the PCR. This assay, combined with a selective culture medium, such as the chromID™ C. difficile, can be applied directly for screening C. difficile-associated disease. The PCR-based assay developed here is rapid (4 h per 21 stool samples) and accurate in diagnosing C. difficile infection, 100 % assay sensitivity and negative predictive value (NPV) were obtained. However, the assay specificity of 99.1 % and positive predictive value (PPV) of 94.9 % were slightly lower than the optimal value of 100 %. The assay protocol outlined here can be used as a rapid screening tool to assist infection control units and in managing infected patients by reducing the number of patients requiring isolation and extended hospitalization. Rapid detection can prevent unnecessary antibiotic therapy and potentially reduce the spread of infection by emerging hypervirulent C. difficile strains. © 2013 SGM.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

62

Issue

9

Start Page

1350

End Page

1356

Number of Pages

7

eISSN

1473-5644

ISSN

0022-2615

Publisher

Microbiology Society

Additional Rights

Free content

Language

en

Peer Reviewed

  • Yes

Open Access

  • No

Acceptance Date

2013-06-15

Era Eligible

  • Yes

Medium

Print-Electronic

Journal

Journal of Medical Microbiology

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