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Process options for conversion of Agave tequilana leaves into bioethanol

journal contribution
posted on 06.12.2017, 00:00 by Deepa Rijal, T Vancov, S McIntosh, Nanjappa Ashwath, Grant Stanley
This paper reports on mild acid pretreatment options for the conversion of Agave tequilana leaves into composite sugars for ethanol fermentation. The effect of five different pretreatment conditions (time, temperature and acid concentrations) were assessed in terms of cellulose digestibility, hemicellulose solubilisation and lignin content in leaves of 1.25 years old A. tequilana plants from Rockhampton and 2.5 year plants from Kalamia. Dilute acid pretreatment and enzyme saccharification of A. tequilana leaf bagasse significantly improved total glucose recovery. A recovery of 273 mg/g (70% theoretical) was attained when the bagasse was pretreated with 2.0% H2SO4 for 60 min at 121 ◦C and saccharified with 6% (w/w) CTec 2.Saccharomyces cerevisiae efficiently fermented crude A. tequilana bagasse and juice hydrolysates within 13 h and 7 h respectively, yielding up to 38.6 g/L and 12.4 g/L. This corresponds to glucose to ethanol conversion rate of 68 and 61% for A. tequilana leaf bagasse and juice, respectively. With further developments, including fermentation of C5 sugars and inulinase saccharification of juices (release of fructose), this process could deliver greater yields, reinforcing its potential as a biofuel feedstock.

Funding

Other

History

Volume

84

Start Page

263

End Page

272

Number of Pages

10

eISSN

1872-633X

ISSN

0926-6690

Location

Netherlands

Publisher

Elsevier

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Department of Primary Industries; Institute for Future Farming Systems (2015- ); School of Medical and Applied Sciences (2013- );

Era Eligible

Yes

Journal

Industrial crops and products.

Exports