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Potential of a novel protein, OMP26, from nontypeable Haemophilus influenzae to enhance pulmonary clearance in a rat model

journal contribution
posted on 06.12.2017, 00:00 by Jennelle Kyd, A Cripps
A major outer membrane protein band of approximately 25 to 27 kDa is commonly observed in strains of Haemophilus influenzae. This study has investigated the potential of a 26-kDa protein (OMP26) from nontypeable H. influenzae (NTHI) as a vaccine candidate. OMP26 was used to immunize rats via intestinal Peyer’s patches, followed by an intratracheal boost. Immunization was found to significantly enhance bacterial clearance following pulmonary challenge with both the homologous NTHI strain and a different NTHI strain. Significant levels of anti-OMP26 were found in the serum and bronchoalveolar lavage from immunized rats, and isotypes of immunoglobulin G (IgG) were also measured in serum. Analysis of IgG isotypes present inserum following OMP26-immunization suggest that predominantly a T-helper 1-type response was induced. The OMP26 protein was amino-terminally sequenced and found to have no homology with the P5 of H.influenzae type b P5 or the fimbrin protein of NTHI, both can migrate upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis at similar molecular masses but OMP26 has 100% homology with a segment ofthe H. influenzae Rd genome. The results of this study suggest that OMP26 may be a suitable vaccine candidate against NTHI infection and warrants continued investigation and characterization.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

66

Issue

5

Start Page

2272

End Page

2278

Number of Pages

7

eISSN

1070-6313

ISSN

0019-9567

Location

United States

Publisher

American Society for Microbiology

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

University of Canberra;

Era Eligible

No

Journal

Infection and immunity.

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