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Piglets cloned from induced pluripotent stem cells

journal contribution
posted on 06.12.2017, 00:00 by N Fan, J Chen, J Han, Q Zhou, D Pan, D Yang, B Zhao, Z Ouyang, Z Liu, Y Zhao, Gabor VajtaGabor Vajta
Embryonic stem (ES) cells are powerful tools for generating genetically modified animals that can assist in advancing our knowledge of mammalian physiology and disease. Pigs provide outstanding models of human genetic diseases due to the striking similarities to human anatomy, physiology and genetics, but progress with porcine genetic engineering has been hampered by the lack of germline-competent pig ES cells. To overcome this limitation, genetically modified pigs have been produced using genetically modified somatic cells and nuclear transfer (NT). Yet, somatic cells exhibit limited proliferative capacity and have an extremely low frequency of homologous recombination compared to ES cells. Hence, only a few knockout pig models have been reported thus far using standard gene-targeting approaches.

Funding

Category 3 - Industry and Other Research Income

History

Volume

23

Issue

1

Start Page

162

End Page

166

Number of Pages

5

eISSN

1748-7838

ISSN

1001-0602

Location

Shanghai China

Publisher

Nature Publishing Group

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

BGI Ark Biotechnology Co Ltd (Shenzen); BGI-Shenzhen; Beijing da xue; Institute for Resource Industries and Sustainability (IRIS); Jilin da xue; Liaoning yi xue yuan; Nan kai da xue (Tianjin, China); Northeast Agricultural University (China); Northwest A & F University; Shangahai Instittues for Biological Sciences; Third Military Medical University; Tong ji da xue (China); Zhejiang da xue; Zhongguo ke xue ji shu da xue; Zhongguo ke xue yuan; Zhongguo nong ye da xue; Zhongguo nong ye ke xue yuan;

Era Eligible

Yes

Journal

Cell research.

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