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Phenolic compounds with antioxidant properties from canola meal extracts inhibit adipogenesis

journal contribution
posted on 04.11.2020, 00:00 by S Hussain, AU Rehman, DJ Luckett, CL Blanchard, HK Obied, Padraig Strappe
The extraction of phenolic compounds from canola meal produces functional health products and renders the canola meal a more digestible animal feed. The extracted phenolics may have novel bioactivity worth investigation. In this study, several solvents were evaluated for their ability to extract phenolic compounds from canola meal: Water (WE) and various 80% organic solvent/water mixtures of methanol (ME), acetone (AE), ethanol (EE), butanol (BE), chloroform (CE) and hexane (HE). The in vitro antioxidant and anti-obesity properties of various extracts were investigated. Anti-obesity properties were studied using adipogenic differentiation inhibition of a murine mesenchymal stem cell line (C3H10T1/2) and a pancreatic lipase inhibition assay. AE, ME, and BE showed significant (p < 0.05) adipogenesis and pancreatic lipase inhibitory activities and may have more pharmacological properties. AE down-regulated the gene expression of the major adipogenic transcription factor, peroxisome proliferator-activated receptor gamma (PPAR), correlating to phenolic content in a dose-dependent manner. The chemical characterization of AE revealed the presence of sinapic acid, ferulic acid, and kaempferol derivatives as main bioactive phenols. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

Other

History

Volume

21

Issue

1

Start Page

1

End Page

17

Number of Pages

17

eISSN

1422-0067

ISSN

1661-6596

Publisher

MDPI AG

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

11/12/2019

Era Eligible

Yes

Journal

International Journal of Molecular Sciences

Usage metrics

CQUniversity

Keywords

Exports