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Novel microsatellite markers for conservation of Australian native Samadera bidwillii

journal contribution
posted on 25.01.2019, 00:00 by Vatsal Naik, Nanjappa Ashwath, RW Lamont, A Shapcott
Microsatellite markers were developed for Samadera bidwillii, a nationally listed vulnerable shrub or small tree, to enable investigation of its genetic structure and diversity within and among populations from its known distribution throughout coastal areas mainly in fragmented and disturbed lands from Mackay to Gympie, Queensland, Australia. The loci were tested for cross-amplification in related Samadera species. Ten polymorphic microsatellite markers were isolated and characterised from an enriched library of S. bidwillii, which exhibited di- and trinucleotide repeats. The mean number of alleles per locus ranged from 1.3 to 2.5 and mean expected and observed heterozygosities ranged from 0.06 to 0.33 and from 0.03 to 0.26, respectively in five populations. All loci successfully amplified in six other closely associated Samadera species also reported from Australia. Developed loci can be used in genetic diversity, population structure and gene flow studies with an emphasis on the conservation of S. bidwillii and related species.

Funding

Category 3 - Industry and Other Research Income

History

Volume

8

Issue

2

Start Page

75

End Page

85

Number of Pages

11

eISSN

2162-1993

ISSN

2162-1985

Publisher

Scientific Research Publishing

Additional Rights

CC BY 4.0

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

08/02/2018

External Author Affiliations

University of the Sunshine Coast

Era Eligible

Yes

Journal

Open Journal of Ecology

Licence

Exports