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Molecular identification and expression of Lyn tyrosine kinase isoforms in marsupials

journal contribution
posted on 06.12.2017, 00:00 by Amy Suthers, Lauren Young
Lyn is a tyrosine kinase molecule required for modulation of signalling cascades in cell populations including B lymphocytes of the mammalian immune system. We have characterised the coding domain of the marsupial lyn gene of two macropod marsupials; the Tammar Wallaby (Macropus eugenii) and the Bridled Nailtail Wallaby (Onychogalea fraenata) and show the co-expression of two Lyn isoforms in cells and tissues of these and three other marsupials (Brush-tail Possum, Trichosurus vulpecula; American Grey Short-tailed Opossum, Monodelphis domestica and Red-tailed Phascogale, Phascogale calura). The predicted Lyn proteins (LynA and LynB) were highly conserved across vertebrate species, with amino acid identities of 94% with their human orthologues and conservation of key tyrosine kinase motifs that suggests that marsupial Lyn most likely functions in cell signalling. Comparison of our cDNA data to annotations for Lyn transcripts (available through the Ensembl Genome Browser) for the Tammar Wallaby confirm splice sites for a number of exons in the wallaby transcript that are missing from the current annotation. This is the first report of the expression of kinase signalling molecules that influence immunity in metatherian mammals and provides key information to support ongoing studies of immune regulation in marsupials.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

55

Issue

3-4

Start Page

310

End Page

318

Number of Pages

9

eISSN

1872-9142

ISSN

0161-5890

Location

United Kingdom

Publisher

Pergamon

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Centre for Environmental Management; Institute for Resource Industries and Sustainability (IRIS); School of Medical and Applied Sciences (2013- );

Era Eligible

Yes

Journal

Molecular immunology.

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