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Molecular characterisation and expression of Interleukin-6 and Interleukin-6Δ2 in the Tammar wallaby (Macropus eugenii)

journal contribution
posted on 2017-12-06, 00:00 authored by Jenifer AlsemgeestJenifer Alsemgeest, J Old, Lauren Young
The pro-inflammatory cytokine, interleukin-6 (IL-6), has not yet been fully characterised in the model macropod, Macropus eugenii, due to incomplete sequence information in publically available genome databases. Using a Rapid Amplification of cDNA Ends strategy we have confirmed the expression and complete nucleotide sequence for this molecule in lymph node tissue and activated leukocytes. Structural conservation of the mature wallaby IL-6 molecule was high when compared with human IL-6, although there was only 34% amino acid sequence identity with the human IL-6 peptide, consistent with reports of evolutionary divergence of this cytokine. We also report the discovery of MeIL-6d2, a splice variant missing exon 2, which directly translates to a truncated non-functional peptide, but which may also code for an alternative peptide that is translated downstream of the canonical IL-6 start site. This putative gene product is predicted to maintain some, if not all, of the functions of macropod IL-6 and is the first IL-6 isoform reported outside of eutherian mammals.

History

Volume

155

Issue

1-2

Start Page

139

End Page

145

Number of Pages

7

eISSN

1873-2534

ISSN

0165-2427

Location

Netherlands

Publisher

Elsevier

Language

en-aus

Peer Reviewed

  • Yes

Open Access

  • No

External Author Affiliations

Centre for Environmental Management; School of Medical and Applied Sciences (2013- ); TBA Research Institute; University of Western Sydney;

Era Eligible

  • Yes

Journal

Veterinary immunology and immunopathology.

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