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Molecular basis for universal HLA-A*0201–restricted CD8+ T-cell immunity against influenza viruses

journal contribution
posted on 02.11.2020, 00:00 by SA Valkenburg, TM Josephs, EB Clemens, EJ Grant, THO Nguyen, GC Wang, DA Price, Adrian MillerAdrian Miller, SYC Tong, PG Thomas
Memory CD8+ T lymphocytes (CTLs) specific for antigenic peptides derived from internal viral proteins confer broad protection against distinct strains of influenza A virus (IAV). However, immune efficacy can be undermined by the emergence of escape mutants. To determine how T-cell receptor (TCR) composition relates to IAV epitope variability, we used ex vivo peptide-HLA tetramer enrichment and single-cell multiplex analysis to compare TCRs targeted to the largely conserved HLA-A∗0201-M158 and the hypervariable HLA-B∗3501-NP418 antigens. The TCRαβs for HLA-B∗3501-NP418+ CTLs varied among individuals and across IAV strains, indicating that a range of mutated peptides will prime different NP418-specific CTL sets. Conversely, a dominant public TRAV27/TRBV19+ TCRαβ was selected in HLA-A∗0201+ donors responding to M158. This public TCR cross-recognized naturally occurring M158 variants complexed with HLA-A∗0201. Ternary structures showed that induced-fit molecular mimicry underpins TRAV27/TRBV19+ TCR specificity for the WT and mutant M158 peptides, suggesting the possibility of universal CTL immunity in HLA-A∗0201+ individuals. Combined with the high population frequency of HLA-A∗0201, these data potentially explain the relative conservation of M158. Moreover, our results suggest that vaccination strategies aimed at generating broad protection should incorporate variant peptides to elicit cross-reactive responses against other specificities, especially those that may be relatively infrequent among IAV-primed memory CTLs.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

113

Issue

16

Start Page

4440

End Page

4445

Number of Pages

6

eISSN

1091-6490

ISSN

0027-8424

Publisher

National Academy of Sciences

Additional Rights

CC-BY

Peer Reviewed

Yes

Open Access

Yes

External Author Affiliations

Menzies School of Health Researc; Cardiff University School of Medicine, UK; University of Melbourne; Monash University; , St Jude Children’s Research Hospital, Johns Hopkins University School of Medicine, USA

Era Eligible

Yes

Journal

Proceedings of the National Academy of Sciences of the United States of America

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