Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells.pdf (5.41 MB)
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Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells

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journal contribution
posted on 12.05.2022, 04:22 by Xuyen T Dinh, Dragana StanleyDragana Stanley, Letitia D Smith, Morgane Moreau, Stuart P Berzins, Adrian Gemiarto, Alan G Baxter, Margaret A Jordan
iNKT cells play a critical role in controlling the strength and character of adaptive and innate immune responses. Their unique functional characteristics are induced by a transcriptional program initiated by positive selection mediated by CD1d expressed by CD4+CD8+ (double positive, DP) thymocytes. Here, using a novel Vα14 TCR transgenic strain bearing greatly expanded numbers of CD24hiCD44loNKT cells, we examined transcriptional events in four immature thymic iNKT cell subsets. A transcriptional regulatory network approach identified transcriptional changes in proximal components of the TCR signalling cascade in DP NKT cells. Subsequently, positive and negative selection, and lineage commitment, occurred at the transition from DP NKT to CD4 NKT. Thus, this study introduces previously unrecognised steps in early NKT cell development, and separates the events associated with modulation of the T cell signalling cascade prior to changes associated with positive selection and lineage commitment.

History

Volume

11

Issue

1

Start Page

1

End Page

23

Number of Pages

23

eISSN

2045-2322

ISSN

2045-2322

Publisher

Nature Research (part of Springer Nature)

Publisher License

CC BY

Additional Rights

CC BY 4.0

Language

en

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

22/11/2021

External Author Affiliations

Federation University Australia; James Cook University

Author Research Institute

Institute for Future Farming Systems

Era Eligible

Yes

Medium

Electronic

Journal

Scientific Reports

Article Number

23650