File(s) not publicly available
Minocycline improves cognition and molecular measures of inflammation and neurodegeneration following repetitive mTBI
journal contribution
posted on 2021-09-16, 23:32 authored by Matthew HiskensMatthew Hiskens, Rebecca VellaRebecca Vella, Anthony SchneidersAnthony Schneiders, Andrew FenningAndrew FenningObjective: To compare the neuroprotective effects of minocycline treatment in a murine model of mTBI on measures of spatial learning and memory, neuroinflammation, excitotoxicity, and neurodegeneration. Design: Adult male C57BL/6 J mice were randomly assigned into vehicle control, vehicle with repetitive mTBI, minocycline without mTBI, or minocycline with repetitive mTBI groups. Methods: A validated mouse model of repetitive impact-induced rotational acceleration was used to deliver 15 mTBIs across 23 days. Cognition was assessed via Morris water maze (MWM) testing, and mRNA analysis investigated MAPT, GFAP, AIF1, GRIA1, TARDBP, TNF, and NEFL genes. Assessment was undertaken 48 h and 3 months following final mTBI. Results: In the chronic phase of recovery, MWM testing revealed impairment in the vehicle mTBI group compared to unimpacted controls (p < .01) that was not present in the minocycline mTBI group, indicating chronic neuroprotection. mRNA analysis revealed AIF1 elevation in the acute cortex (p < .01) and chronic hippocampus (p < .01) of the vehicle mTBI group, with minocycline treatment leading to improved markers of microglial activation and inflammation in the chronic stage of recovery. Conclusions: These data suggest that minocycline treatment alleviated some mTBI pathophysiology and clinical features at chronic time-points.
History
Volume
35Issue
7Start Page
831End Page
841Number of Pages
11eISSN
1362-301XISSN
0269-9052Location
EnglandPublisher
Taylor & FrancisPublisher DOI
Full Text URL
Language
engPeer Reviewed
- Yes
Open Access
- No
Acceptance Date
2021-03-15Era Eligible
- Yes
Medium
Print-ElectronicJournal
Brain InjuryUsage metrics
Keywords
concussionanimal modelsneurobehavioralrehabilitationHippocampusAnimalsMice, Inbred C57BLMiceDisease Models, AnimalInflammationMinocyclineCognitionMaze LearningMaleMedical and health sciencesPsychology and cognitive sciencesClinical Pharmacology and TherapeuticsRehabilitation and Therapy (excl. Physiotherapy)