File(s) not publicly available

Minocycline improves cognition and molecular measures of inflammation and neurodegeneration following repetitive mTBI

Objective: To compare the neuroprotective effects of minocycline treatment in a murine model of mTBI on measures of spatial learning and memory, neuroinflammation, excitotoxicity, and neurodegeneration. Design: Adult male C57BL/6 J mice were randomly assigned into vehicle control, vehicle with repetitive mTBI, minocycline without mTBI, or minocycline with repetitive mTBI groups. Methods: A validated mouse model of repetitive impact-induced rotational acceleration was used to deliver 15 mTBIs across 23 days. Cognition was assessed via Morris water maze (MWM) testing, and mRNA analysis investigated MAPT, GFAP, AIF1, GRIA1, TARDBP, TNF, and NEFL genes. Assessment was undertaken 48 h and 3 months following final mTBI. Results: In the chronic phase of recovery, MWM testing revealed impairment in the vehicle mTBI group compared to unimpacted controls (p < .01) that was not present in the minocycline mTBI group, indicating chronic neuroprotection. mRNA analysis revealed AIF1 elevation in the acute cortex (p < .01) and chronic hippocampus (p < .01) of the vehicle mTBI group, with minocycline treatment leading to improved markers of microglial activation and inflammation in the chronic stage of recovery. Conclusions: These data suggest that minocycline treatment alleviated some mTBI pathophysiology and clinical features at chronic time-points.

History

Volume

35

Issue

7

Start Page

831

End Page

841

Number of Pages

11

eISSN

1362-301X

ISSN

0269-9052

Location

England

Publisher

Taylor & Francis

Language

eng

Peer Reviewed

Yes

Open Access

No

Acceptance Date

15/03/2021

Era Eligible

Yes

Medium

Print-Electronic

Journal

Brain Injury