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Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the bio-Quant Direct ELISA presumptive assays for amphetamine and methamphetamine
journal contribution
posted on 2017-12-06, 00:00 authored by L Apollonio, I Whittall, D Pianca, Jennelle KydJennelle Kyd, W MaherThe aim of this study was to evaluate the Bio-Quant Direct ELISAassays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3–15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing.
Funding
Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)
History
Volume
31Start Page
208End Page
213Number of Pages
6ISSN
0146-4760Location
United StatesPublisher
PrestonLanguage
en-ausPeer Reviewed
- Yes
Open Access
- No
External Author Affiliations
Government Analytical Laboratory; University of Canberra;Era Eligible
- Yes