File(s) not publicly available

Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the bio-Quant Direct ELISA presumptive assays for amphetamine and methamphetamine

journal contribution
posted on 06.12.2017, 00:00 by L Apollonio, I Whittall, D Pianca, Jennelle KydJennelle Kyd, W Maher
The aim of this study was to evaluate the Bio-Quant Direct ELISAassays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3–15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

31

Start Page

208

End Page

213

Number of Pages

6

ISSN

0146-4760

Location

United States

Publisher

Preston

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Government Analytical Laboratory; University of Canberra;

Era Eligible

Yes

Journal

Journal of analytical toxicology.

Usage metrics

Exports