Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the bio-Quant Direct ELISA presumptive assays for amphetamine and methamphetamine
journal contribution
posted on 2017-12-06, 00:00authored byL Apollonio, I Whittall, D Pianca, Jennelle Kyd, W Maher
The aim of this study was to evaluate the Bio-Quant Direct ELISAassays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3–15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing.
Funding
Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)
History
Volume
31
Start Page
208
End Page
213
Number of Pages
6
ISSN
0146-4760
Location
United States
Publisher
Preston
Language
en-aus
Peer Reviewed
Yes
Open Access
No
External Author Affiliations
Government Analytical Laboratory; University of Canberra;