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Live birth from vitrified-warmed human oocytes fertilized with frozen-thawed testicular spermatozoa

journal contribution
posted on 2017-12-06, 00:00 authored by S Liow, L Foong, N Chen, W Yip, C Khaw, J Kumar, Gabor VajtaGabor Vajta, S Ng
A couple with male infertility due to non-obstructive azoospermia were referred to the fertility centre for treatment. Testicular biopsy was performed on the male partner and testicular samples were frozen. The female partner underwent ovarian stimulation and 31 mature oocytes were recovered by ultrasound-guided vaginal aspiration. Twelve oocytes were cryopreserved by the Cryotop vitrification method and 19 oocytes were inseminated by intracytoplasmic sperm injection (ICSI) using frozen–thawed testicular spermatozoa. Nine out of 19 oocytes were fertilized and the resulting embryos were cryopreserved by slow freezing. Four months later, two out of six thawed embryos were transferred, but no pregnancy resulted. One year later, the couple decided to attempt pregnancy using vitrified oocytes and frozen testicular spermatozoa. Six vitrified–warmed oocytes were injected with frozen–thawed testicular spermatozoa and four were fertilized. On the day of transfer, two cleavage stage embryos (4-cell, 2-cell) were obtained. Serum β-HCG test 14 days after embryo transfer was positive. Hormonal support for the established pregnancy was maintained with oestradiol and progesterone orally until 12 weeks of gestation. A healthy baby boy weighing 3.09 kg was delivered by elective Caesarean section at 38 weeks of gestation. This case report demonstrates that oocyte cryopreservation by the Cryotop vitrification method does not compromise oocyte developmental competence.

History

Volume

19

Issue

2

Start Page

198

End Page

201

Number of Pages

4

eISSN

1472-6491

ISSN

1472-6483

Location

United Kingdom

Publisher

Elsevier Ltd

Language

en-aus

Peer Reviewed

  • Yes

Open Access

  • No

External Author Affiliations

Embryonics International; O and G Partners Fertility Centre; PIVET Medical Centre, Perth; TBA Research Institute;

Era Eligible

  • Yes

Journal

Reproductive biomedicine online.

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