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Investigation of mucosal immunisation in pulmonary clearance of Moraxella (Branhamella) catarrhalis

journal contribution
posted on 06.12.2017, 00:00 by Jennelle KydJennelle Kyd, A John, A Cripps, T Murphy
Moraxella (Branhamella) catarrhalis is a common cause of otitis media in children and respiratory infection in adults with chronic obstructive pulmonary disease. To identify immune responses that may facilitate the development of a mucosal vaccine, a mouse model to study pulmonary responses was established. Regimes involving intra-Peyer's patch, intratracheal and intranasal routes of immunisation with killed M. catarrhalis were investigated. A mucosal immunisation regime of a primary intra-Peyer's patch immunisation with an intratracheal boost resulted in significantly enhanced pulmonary clearance of bacteria compared to controls following an intratracheal challenge with live bacteria. Additional intratracheal boosts did not induce further enhancement of clearance. Intra-Peyer's patch immunisation alone, intratracheal and intranasal immunisations did notinduce enhanced clearance. The levels of specific IgG and IgA in serum and bronchoalveolar lavage ¯uid correlated with pulmonary clearance. The present study showed that mucosal immunisation induced enhanced pulmonary clearance of M. catarrhalis following live bacterial challenge. This mucosal immunisation model has demonstrated that a mucosal vaccine, particularly an oral vaccine, would be feasible.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

18

Start Page

398

End Page

406

Number of Pages

9

eISSN

1873-2518

ISSN

0264-410X

Location

United Kingdom

Publisher

Elsevier Ltd

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

State University of New York at Buffalo; University of Canberra; University of Canberra;

Era Eligible

No

Journal

Vaccine.

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