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Investigation of AGID and two commercial ELISAs for the detection of Bovine viral diarrhea virus-specific antibodies in sheep serum.
journal contribution
posted on 2023-03-08, 03:40 authored by Caitlin Evans, Sasha R Lanyon, Michael P ReichelEffective control and the eventual eradication of Bovine viral diarrhea virus (BVDV) from cattle populations depend on the accurate identification of infected animals. Although typically a disease agent of cattle, BVDV is known to infect a wide variety of nonbovine species, including sheep. However, validation of serologic tests in these nonbovine species, particularly sheep, is lacking. We analyzed 99 sheep sera (57 samples from Pestivirus-naive sheep, and 42 samples from BVDV-inoculated sheep) in order to investigate 3 serologic tests: the agarose gel immunodiffusion (AGID) and 2 commercial enzyme-linked immunosorbent assays (ELISAs) for detection of BVDV antibodies. At the manufacturer's cutoff thresholds, the AGID performed with 95.2% diagnostic sensitivity; ELISA-A performed with sensitivity of 90.5% and ELISA-B with 69.1%. All 3 tests performed with 100% diagnostic specificity. Two-graph receiver operating characteristic analysis showed that performance characteristics were optimized, such that both diagnostic sensitivity and diagnostic specificity were >95% for both ELISAs, if the thresholds were altered to 34.9% inhibition for ELISA-A and 63.5 signal-to-noise ratio for ELISA-B.
History
Volume
29Issue
2Start Page
181End Page
185Number of Pages
5eISSN
1943-4936ISSN
1040-6387Location
United StatesPublisher
SAGEPublisher DOI
Language
engPeer Reviewed
- Yes
Open Access
- No
External Author Affiliations
University of Hong Kong; University of AdelaideAuthor Research Institute
- Institute for Future Farming Systems
Era Eligible
- Yes