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In vitro culture of immature seed for rapid generation advancement in tomato

journal contribution
posted on 06.12.2017, 00:00 authored by Surya BhattaraiSurya Bhattarai, R De La Pena, David MidmoreDavid Midmore, K Palchamy
The importance of fast-trackt generation advancement in developing superior germplasm has been recognized in breeding of many crop species. To address this issue in tomato, immature seeds were excised from fruit at different maturity stages and transferred to culture medium. The best culture medium was modified full strength Moorashige–Skoog (MS) salts supplemented with 0.1 mg l-¹IAA, 0.5 mg l-¹ IBA, 0.5 mg l-¹ GA3 and 2% sucrose. If the excised seeds were able to grow, most showed shoot formation after a week. Seeds extracted as early as 10 days after pollination were successfully cultured provided they were transferred aseptically and without injury. No morphological or physiological changes in regenerated plants and their fruit relative to the parent were detected. Germination from immature seeds of tomato is a simpler alternative to in vitro culture of immature embryos or callus, as it can be undertaken in comparatively less stringent laboratory conditions. Using this approach, five generations can be produced in a year in contrast to a maximum of three generations with conventional methods. This offers an opportunity for rapid generation advancement aimed towards population development when coupled with marker assisted selection in tomato breeding for biotic and abiotic stress tolerance.

Funding

Category 4 - CRC Research Income

History

Volume

167

Issue

1

Start Page

23

End Page

30

Number of Pages

8

eISSN

1573-5060

ISSN

0014-2336

Location

Netherlands

Publisher

Springer

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Asian Vegetable Research and Development Center; Centre for Plant and Water Science; Institute for Resource Industries and Sustainability (IRIS);

Era Eligible

Yes

Journal

Euphytica.