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In vitro culture of immature seed for rapid generation advancement in tomato
journal contribution
posted on 2017-12-06, 00:00 authored by Surya BhattaraiSurya Bhattarai, R De La Pena, David MidmoreDavid Midmore, K PalchamyThe importance of fast-trackt generation advancement in developing superior germplasm has been recognized in breeding of many crop species. To address this issue in tomato, immature seeds were excised from fruit at different maturity stages and transferred to culture medium. The best culture medium was modified full strength Moorashige–Skoog (MS) salts supplemented with 0.1 mg l-¹IAA, 0.5 mg l-¹ IBA, 0.5 mg l-¹ GA3 and 2% sucrose. If the excised seeds were able to grow, most showed shoot formation after a week. Seeds extracted as early as 10 days after pollination were successfully cultured provided they were transferred aseptically and without injury. No morphological or physiological changes in regenerated plants and their fruit relative to the parent were detected. Germination from immature seeds of tomato is a simpler alternative to in vitro culture of immature embryos or callus, as it can be undertaken in comparatively less stringent laboratory conditions. Using this approach, five generations can be produced in a year in contrast to a maximum of three generations with conventional methods. This offers an opportunity for rapid generation advancement aimed towards population development when coupled with marker assisted selection in tomato breeding for biotic and abiotic stress tolerance.
Funding
Category 4 - CRC Research Income
History
Volume
167Issue
1Start Page
23End Page
30Number of Pages
8eISSN
1573-5060ISSN
0014-2336Location
NetherlandsPublisher
SpringerPublisher DOI
Full Text URL
Language
en-ausPeer Reviewed
- Yes
Open Access
- No
External Author Affiliations
Asian Vegetable Research and Development Center; Centre for Plant and Water Science; Institute for Resource Industries and Sustainability (IRIS);Era Eligible
- Yes