Improving the quality of in vitro cultured shoots of tomato (Lycopersicon esculentum mill. cv. red coat)

Effects of activated charcoal (0,1,5 and 10 mg L-1), ascorbic acid (30, 60, 120, 240 and 480 uM) and casein hydrolysate (0, 50, 100, 200, 500 and 1000 mg L-1) were evaluated for morphogenesis of Lycopersicon esculentum cv. Red Coat cotyledons. The media containing higher concentrations of activated charcoal and ascorbic acid produced longer shoots. Addition of casein hydrolysate significantly reduced callus response.