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IGF-1 splice variant and IGF-1 peptide expression patterns in young and old human skeletal muscle prior to and following sequential exercise bouts

journal contribution
posted on 06.12.2017, 00:00 by M Roberts, Vincent DalboVincent Dalbo, K Sunderland, C Poole, S Hassell, D Bemben, J Cramer, J Stout, C Kerksick
Genes and proteins involved in insulin-like growth factor (IGF)-1 signaling are thought to be differentially expressed in older versus younger mammalian skeletal muscle following acute exercise. The purpose of this study was to examine how multiple bouts of conventional resistance training meant to elicit hypertrophy affect the mRNA expression of IGF-1EA and IGF-1EC (MGF) as well as the expression of total IGF-1 peptides in human skeletal muscle. Ten younger (18–25 years) and 10 older (60–75 years) males completed three sequential workouts (M, W, F) consisting of nine sets of lower body exercises with ten repetitions per set at an intensity of 80% of one repetition maximum. Vastus lateralis muscle biopsies were collected prior to intervention (T1), 48 h following workout 1 (T2), 48 h following workout 2 (T3), and 24 h following workout 3 (T4). RT-PCR was performed to assess baseline and changes in MGF and IGF-IEA mRNA. Samples were also assayed for total muscle IGF-1 peptides using ELISA-based methods. There were no baseline differences in MGF or IGF-1EA mRNA expression and IGF-1 peptides between age groups. Interestingly, MGF expression increased at T2–T4 in the older group relative to baseline values (p < 0.05), albeit muscle IGF-1EA mRNA.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

110

Issue

5

Start Page

961

End Page

969

Number of Pages

9

eISSN

1439-6327

ISSN

1439-6319

Location

Dusseldorf, Germany

Publisher

Springer

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Faculty of Sciences, Engineering and Health; Institute for Health and Social Science Research (IHSSR); University of Oklahoma;

Era Eligible

Yes

Journal

European journal of applied physiology.

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