OBJECTIVE: This study investigated the association between COVID-19 prevention knowledge and concern and practising preventive behaviour in Australian adults. METHODS: Using an online survey, knowledge of Australian COVID-19 guidelines, concerns about pandemic impact, the practice of preventive behaviours, and sociodemographic variables (i.e. age, gender, information source) were measured. Bivariate analysis and linear regression models were used. RESULTS: A total of 1,491 participants (age 50.5 ±14.9 years, 32.3% males) completed the survey. Higher knowledge and concern scores were associated with a higher practice of preventive behaviour scores (βs:0.47 & 0.08 respectively, p<0.001). Older adults (>65 years) and women had higher knowledge and practice scores compared to their counterparts. Being younger (<45 years) and male were associated with a lower practice score (βs:-0.88 & -2.52, respectively, p<0.001). Referring to public and government sources as primary sources of information was associated with a higher practice score (β:1.21, p<0.001). CONCLUSIONS: Government-run campaigns appear to be effective in promoting preventive practices and achieving a high knowledge of COVID-19 guidelines in Australian adults. Implications for public health: Public health strategies are required to promote the practice of preventive behaviour for COVID-19 (or future pandemics), especially among men and younger adults using social media, given their wide use of these sources.