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High in vitro development after somatic cell nuclear transfer and trichostatin : a treatment of reconstructed porcine embryos

journal contribution
posted on 06.12.2017, 00:00 by J Li, O Svarcova, P Maddox-Hyttel, Gabor VajtaGabor Vajta, K Villemoes, P Kragh, M Schmidt, I Bøgh, X Zhang, L Lin
Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer (SCNT). Trichostatin A (TSA) is an inhibitor of histone deacetylase, potentially enhancing cloning efficiency. The aim of our present study was to establish the optimal TSA treatment in order to improve the development of handmade cloned (HMC) porcine embryos and examine the effect of TSA on their development. The blastocyst percentage of HMC embryos treated with 37.5 nM TSA for 22–24 h after activation increased up to 80% (control group-54%; P < 0.05). TSA mediated increase in histone acetylation was proved by immunofluorescence analysis of acH3K9 and acH4K16. 2-cell stage embryos derived from TSA treatment displayed significant increase in histone acetylation compared to control embryos, whereas no significant differences were observed at blastocyst stage. During time-lapse monitoring, no difference was observed in the kinetics of 2-cell stage embryos. Compact morula (CM) stage was reached 15 h later in TSA treated embryos compared to the control. Blastocysts (Day 5 and 6) from HMC embryos treated with TSA were transferred to 2 recipients resulting in one pregnancy and birth of one live and five dead piglets. Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development.

History

Volume

70

Issue

5

Start Page

800

End Page

808

Number of Pages

9

eISSN

1879-3231

ISSN

0093-691X

Location

USA

Publisher

Elsevier Inc.

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Aarhus universitet; Anhui Agricultural University, China; Københavns universitet; PIVET Medical Centre, Perth; TBA Research Institute; Univerzita Konštantína Filozofa; Zhejiang da xue; Zhongguo ke xue yuan;

Era Eligible

Yes

Journal

Theriogenology.