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Genome-wide mapping of ZNF652 promoter binding sites in breast cancer cells

journal contribution
posted on 25.10.2018, 00:00 by R Kumar, LA Selth, RB Schulz, BS Tay, Paul Neilsen, DF Callen
A significant proportion of transcription factors encoded by the human genome are classical C 2 H 2 zinc finger proteins that regulate gene expression by directly interacting with their cognate DNA binding motifs. We previously showed that one such C 2 H 2 zinc finger DNA binding protein, ZNF652 (zinc finger protein 652), specifically and functionally interacts with CBFA2T3 to repress transcription of genes involved in breast oncogenesis. To identify potential targets by which ZNF652 exerts its putative tumour suppressive function, its promoter-specific cistrome was mapped by ChIP-chip. De novo motif scanning of the ZNF652 binding sites identified a novel ZNF652 recognition motif that closely resembles the previously characterised in vitro binding site, being a 10 nucleotide core of that 13 nucleotide sequence. Genes with ZNF652 binding sites function in diverse cellular pathways, and many are involved in cancer development and progression. Characterisation of the in vivo ZNF652 DNA binding motif and identification of potential ZNF652 target genes are key steps towards elucidating the function(s) of this transcription factor in the normal and malignant breast cell. Copyright © 2011 Wiley-Liss, Inc.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

112

Issue

10

Start Page

2742

End Page

2747

Number of Pages

6

eISSN

1097-4644

ISSN

0730-2312

Publisher

John Wiley & Sons, Inc.

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

University of Adelaide and Hanson Institute

Era Eligible

Yes

Journal

Journal of Cellular Biochemistry

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