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Genetic analysis of Giardia and Cryptosporidium from people in Northern Australia using PCR-based tools

journal contribution
posted on 2017-12-06, 00:00 authored by J Ebner, A Koehler, G Robertson, Richard Bradbury, A Jex, S Haydon, M Stevens, R Norton, A Joachim, R Gasser
To date, there has been limited genetic study of the gastrointestinal pathogens Giardia and Cryptosporidiumin northern parts of Australia. Here, PCR-based methods were used for the genetic characterization of Giardiaand Cryptosporidium from 695 people with histories of gastrointestinal disorders from the tropical North of Australia. Genomic DNAs from fecal samples were subjected to PCR-based analyses of regions from the triose phosphate isomerase (tpi), small subunit (SSU) of the nuclear ribosomal RNA and/or the glycoprotein (gp60) genes. Giardia and Cryptosporidium were detected in 13 and four of the 695 samples, respectively. Giardia duodenalis assemblages A and B were found in 4 (31%) and 9 (69%) of the 13 samples in persons of <9 years of age. Cryptosporidium hominis (subgenotype IdA18), Cryptosporidium mink genotype (subgenotype IIA16R1) and C. felis were also identified in single patients of 11–21 years of age. Future studies might focus on a comparative study of these and other protists in rural communities in Northern Australia.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

36

Start Page

389

End Page

395

Number of Pages

7

eISSN

1567-7257

ISSN

1567-1348

Location

Netherlands

Publisher

Elsevier

Language

en-aus

Peer Reviewed

  • Yes

Open Access

  • No

External Author Affiliations

Melbourne Water Corporation; School of Medical and Applied Sciences (2013- ); TBA Research Institute; Townsville Hospital (Qld.); University of Melbourne; Veterinärmedizinische Universität Wien;

Era Eligible

  • Yes

Journal

Infection, genetics and evolution.