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Functional enrichment of mannanase-treated spent brewer yeast

journal contribution
posted on 22.05.2018, 00:00 by R Cao, X Yang, W Shang, Z Zhou, Padraig Strappe, C Blanchard
In this study, the effect of Bacillus amyloliquefaciens-produced β-mannanase on the nutrient diffusion (release) and antioxidant activity of spent brewer yeast (SBY) was investigated. Three pretreatments were performed: (1) autolysis at 50°C for 24 h; (2) autolysis at 50°C for 24 h, with the addition of β-mannanase during the autolysis; (3) autolysis at 50°C for 24 h, and the β-mannanase was added for another 12 h treatment. The pretreatments with the addition of β-mannanase caused significant cell wall degradation, markedly increased the yield of SBY extracts. More importantly, this study found that polysaccharides were degraded to be oligosaccharides with a considerable reduction in molecular weights. Meanwhile, pretreatment with the enzyme also exhibited a higher antioxidant activity in SBY extract compared to autolysis itself. The current study indicated that pretreatment (3) had a better effect than pretreatment (2) in terms of improving in antioxidant activity in SBY extract. These improved characteristics of SBY extracts isolated through enzymatic treatment appear to show promising features for their prospective use as natural functional agents. © 2017 Taylor & Francis.

Funding

Other

History

Volume

47

Issue

8

Start Page

789

End Page

794

Number of Pages

6

eISSN

1532-2297

ISSN

1082-6068

Peer Reviewed

Yes

Open Access

No

Acceptance Date

21/06/2017

External Author Affiliations

Tianjin University of Science and Technology, Tianjin, China; Ministry of Education, Tianjin, China; Charles Sturt University

Era Eligible

Yes

Journal

Preparative Biochemistry and Biotechnology

Exports