Functional design of electrochemical biosensors using polypyrrole

In order to improve biosensor performance namely, the sensitivity, response time, and linear range values, novel amerometric polypyrrole (PPy) glucose biosensors, achieved by three different immobilizing techniques namely, physical adsorption, coentrapment and a two steps method of coentrapment superimposed with physical adsorption had been studied. Different enzyme loading and material properties resulted in varied immobilizing techniques lead to dissimilarities in characteristics.