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Feasibility study on the use of attenuated total reflectance infrared spectroscopy as high throughput screening tool to phenotype single barley seeds (Hordeum vulgare L.)
journal contribution
posted on 2018-09-19, 00:00 authored by Daniel CozzolinoDaniel Cozzolino, S Roumeliotis, J EglintonKnowledge of the chemical and structural differences between barley (Hordeum vulgare L.) varieties and breeding lines might lead to an understanding of the reasons for these differences among genotypes in relation to their malting properties. The aim of this study was to evaluate the use of attenuated total reflectance mid infrared (ATR-MIR) spectroscopy combined with univariate and multivariate methods as a high throughput screening tool to phenotype single seeds of barley. Varieties that yield high malting quality (hot water extract (HWE) > 80%) showed relatively high non-structural carbohydrates (e.g. starch) to protein and non-structural to structural (e.g. cellulose) carbohydrates ratios compared with those samples having moderate HWE (78% < HWE < 80%). The use of ATR-MIR spectroscopy and in particular the use of ratios at specific wavenumbers can provide useful information about the biophysical and chemical characteristics of the grain.
Funding
Category 2 - Other Public Sector Grants Category
History
Volume
116Issue
4Start Page
379End Page
384Number of Pages
6ISSN
1537-5110Publisher
ElsevierPublisher DOI
Peer Reviewed
- Yes
Open Access
- No
Acceptance Date
2013-08-11External Author Affiliations
The University of AdelaideEra Eligible
- Yes