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Feasibility study on the use of attenuated total reflectance MIR spectroscopy to measure the fructan content in barley

journal contribution
posted on 05.09.2018, 00:00 by Daniel CozzolinoDaniel Cozzolino, S Roumeliotis, J Eglinton
The aim of this study was to evaluate the feasibility of using attenuated total reflectance mid-infrared (ATR-MIR) spectroscopy to predict the fructan content in both barley and malt flour samples. Samples (n = 60) were sourced from commercial and experimental barley grain varieties and their corresponding malts. The fructan content in grain and malt flour was determined using the enzymatic kit from Megazyme (K-FRUC, Megazyme International Ireland). Samples were scanned in a MIR instrument using an ATR single bounce cell (Bruker Optics, Germany). The coefficients of determination in cross-validation (R2) and the standard error of cross-validation (SECV) obtained for the prediction of the fructan content in the calibration set were 0.76 and 0.20%, respectively. The residual predictive deviation (RPD = SD/SECV) value obtained was 2.3, indicating that these calibrations can be used for qualitative determination of the fructan content (e.g. low, medium and high) in the set of samples analysed. This study showed that ATR-MIR spectroscopy might be used as approximate estimates of the true fructan concentration in barley and malt in order to rank samples (low, medium, high) in the context of a breeding program. This journal is © the Partner Organisations 2014.

Funding

Other

History

Volume

6

Issue

19

Start Page

7710

End Page

7715

Number of Pages

6

eISSN

1759-9679

ISSN

1759-9660

Publisher

Royal Society of Chemistry, UK

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

The University of Adelaide

Era Eligible

Yes

Journal

Analytical Methods