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Experimental design considerations in microbiota/inflammation studies

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journal contribution
posted on 08.02.2022, 02:53 authored by RJ Moore, Dragana StanleyDragana Stanley
There is now convincing evidence that many inflammatory diseases are precipitated, or at least exacerbated, by unfavourable interactions of the host with the resident microbiota. The role of gut microbiota in the genesis and progression of diseases such as inflammatory bowel disease, obesity, metabolic syndrome and diabetes have been studied both in human and in animal, mainly rodent, models of disease. The intrinsic variation in microbiota composition, both within one host over time and within a group of similarly treated hosts, presents particular challenges in experimental design. This review highlights factors that need to be taken into consideration when designing animal trials to investigate the gastrointestinal tract microbiota in the context of inflammation studies. These include the origin and history of the animals, the husbandry of the animals before and during experiments, details of sampling, sample processing, sequence data acquisition and bioinformatic analysis. Because of the intrinsic variability in microbiota composition, it is likely that the number of animals required to allow meaningful statistical comparisons across groups will be higher than researchers have generally used for purely immune-based analyses.

History

Volume

5

Start Page

1

End Page

7

Number of Pages

7

ISSN

2050-0068

Location

England

Publisher

Nature Publishing Group

Additional Rights

Clinical & Translational Immunology is an open access journal: authors pay an article processing charge (APC) for their accepted articles to be open access online and freely accessible immediately upon publication, under a Creative Commons license.

Language

eng

Peer Reviewed

Yes

Open Access

Yes

Acceptance Date

06/06/2016

External Author Affiliations

RMIT University; Monash University

Era Eligible

Yes

Journal

Clinical and Translational Immunology