Spectral indices can be used in assessment of pigment level of intact plant material, and thus there exists the possibly of using spectral indices as a measure of fruit maturity and ripeness. Spectra of tomato, banana and mango fruit were collected with a Nirvana handheld unit (Integrated Spectronics, Australia). A number of spectral indices: greenness index = R554/R677; DA index = A670-A720; chlorophyll index = R800/R700-1; Car/Chl ratio = (R678-R500)/R750; chlorophyll ratio = (R750/R700) and xanthophyll index = (R530-R570)/(R530+R570) were assessed. Tomato maturity was best discriminated using the Tomato index and carotene/chlorophyll ratio, while mango maturation and banana ripening were best indexed by DA index and chlorophyll ratio.