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Elemental mapping using PIXE shows the main pathway of nickel movement is principally symplastic within the fruit of the hyperaccumulator Stackhousia tryonii

journal contribution
posted on 06.12.2017, 00:00 by Naveen BhatiaNaveen Bhatia, I Orlic, R Siegler, Nanjappa AshwathNanjappa Ashwath, A Baker, Kerry WalshKerry Walsh
Metal concentrations within reproductive tissues of metallophytes are rarely reported. Here, the spatial distribution of nickel (Ni) within the fruits (seeds) of the Ni hyperaccumulator Stackhousia tryonii was investigated. Two microanalytical techniques, energy dispersive x-ray spectrometry (EDXS) and nuclear microprobe (micro-proton-induced x-ray emission spectrometry; micro-PIXE) were employed for qualitative and quantitative assessment, respectively, of localized Ni, within the fruits of S. tryonii The results were compared with quantitative analysis made using inductively coupled plasma-optical emission spectrometry (ICP-OES). Nickel analysis made using micro-PIXE was consistent with bulk (ICP-OES) analysis (at 1800 μg g − 1 d. wt), however, a beam resolution of approx. 2 × 2 μm 2 allowed tissue localization. Nickel was partitioned to the fruit wall (pericarp) (4433 μg g − 1 ), while endospermic and cotyledonary tissues possessed little Ni (309 and 182 μg g − 1 d. wt, respectively). This distribution is consistent with the interpretation that principal pathway of Ni movement within the fruit is symplastic rather than apoplastic (as the filial generation lacks symplastic connection with the parent).

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

160

Issue

3

Start Page

479

End Page

488

Number of Pages

10

ISSN

0028-646X

Location

United Kingdom

Publisher

Blackwell Publishing Ltd

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Australian Nuclear Science and Technology Organisation; Faculty of Arts, Health and Sciences; School of Botany; TBA Research Institute;

Era Eligible

Yes

Journal

New phytologist.