Electrophoretic enzyme deposition on nano-structured conducting polymer substrates for high sensitivity biosensors

Performance of enzyme based biosensors directly depends on how much enzyme can be loaded onto a unit surface area of the sensor substrate, and how easily the particles under measurement reach the substrate. In the past we have developed nano-structured substrate surfaces using conducting polymer Polypyrrole (PPy) to increase the effective sensor surface area for a unit 2-D area and have developed very high enzyme adsorption using Gluteraldehyde as cross-linker [1],[2]. In this work we have developed a novel method of further improving enzyme adsorption. The enzyme was dissolved in a phosphate buffer solution and was allowed to adsorb under a high electric field.