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Efficient transduction of equine adipose-derived mesenchymal stem cells by VSV-G pseudotyped lentiviral vectors

journal contribution
posted on 15.08.2018, 00:00 authored by GF Petersen, B Hilbert, G Trope, W Kalle, Padraig Strappe
Equine adipose-derived mesenchymal stem cells (EADMSC) provide a unique cell-based approach for treatment of a variety of equine musculoskeletal injuries, via regeneration of diseased or damaged tissue, or the secretion of immunomodulatory molecules. These capabilities can be further enhanced by genetic modification using lentiviral vectors, which provide a safe and efficient method of gene delivery. We investigated the suitability of lentiviral vector technology for gene delivery into EADMSC, using GFP expressing lentiviral vectors pseudotyped with the G glycoprotein from the vesicular stomatitis virus (V-GFP) or, for the first time, the baculovirus gp64 envelope protein (G-GFP). In this study, we produced similarly high titre V-GFP and G-GFP lentiviral vectors. Flow cytometric analysis showed efficient transduction using V-GFP; however G-GFP exhibited a poor ability to transduce EADMSC. Transduction resulted in sustained GFP expression over four passages, with minimal effects on cell viability and doubling time, and an unaltered chondrogenic differentiation potential.

Funding

Other

History

Volume

97

Issue

3

Start Page

616

End Page

622

Number of Pages

7

eISSN

1532-2661

ISSN

0034-5288

Publisher

Elsevier, Uk

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Charles Sturt University

Era Eligible

Yes

Journal

Research in Veterinary Science