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Effects of pre-exercise feeding on serum hormone concentrations and biomarkers of myostatin and ubiquitin proteasome pathway activity

journal contribution
posted on 06.12.2017, 00:00 by Vincent DalboVincent Dalbo, M Roberts, S Hassell, C Kerksick
Purpose: The aim of the study was to examine the acute effects of pre-exercise ingestion of protein, carbohydrate, and a non-caloric placebo on serum concentrations of insulin and cortisol, and the intramuscular gene expression of myostatin- and ubiquitin proteasome pathway (UPP)- related genes following a bout of resistance exercise. Methods: Ten untrained college-aged men participated in three resistance exercise sessions (3 x 10 at 80 % 1RM for bilateral hack squat, leg press, and leg extension) in a crossover fashion, which were randomly preceded by protein, carbohydrate, or placebo ingestion 30 min prior to training. Pre-supplement/pre-exercise, 2 h and 6 h post-exercise muscle biopsies were obtained during each session and analyzed for mRNA fold changes in myostatin (MSTN), activin IIB, follistatin-like 3 (FSTL3), SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), forkhead box O3, F-box protein 32 (FBXO32), and Muscle RING-finger protein-1, with beta-actin serving as the housekeeping gene. Gene expression of all genes was analyzed using real-time PCR. Results: Acute feeding appeared to have no significant effect on myostatin or UPP biomarkers. However, resistance exercise resulted in a significant downregulation of MSTN and FBXO32 mRNA expression and a significant upregulation in FSTL3 and SMURF1 mRNA expression (p<0.05). Conclusions: An acute bout of resistance exercise results in acute post-exercise alterations in intramuscular mRNA expression of myostatin and UPP markers suggestive of skeletal muscle growth. However, carbohydrate and protein feeding surrounding resistance exercise appear to have little influence on the acute expression of these markers.

History

Volume

52

Issue

2

Start Page

477

End Page

487

Number of Pages

11

eISSN

1436-6215

ISSN

1436-6207

Location

Germany

Publisher

Springer Medizin

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Dept. of Biomedical Science; Institute for Health and Social Science Research (IHSSR); Institute for Health and Social Science Research (IHSSR); University of Oklahoma;

Era Eligible

Yes

Journal

European journal of nutrition.