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Effects of endurance training on intracellular calcium concentration in T lymphocytes

journal contribution
posted on 06.12.2017, 00:00 by S Broadbent, Gregory Gass
The purpose of the present study was to determine whether 12 months of endurance training reduced [Ca2+]i in T helper (CD4+) lymphocytes intrained (TR) men compared to untrained (UT). Fourteen trained (Ironman triathletes) and nine untrained (sedentary) men volunteered for the study. The TR group averaged 12 km of swimming, 300 km of cycling and 60 km of running per week during the year. Resting blood samples were taken from TR (VO2peak 64 ± 2 ml kg -1 min -1) and UT (VO2peak 42 ± 2 mlkg -1 min -1) subjects every 4 weeks for 52 weeks (October 1, 1999-October 1, 2000). Leukocyte concentration was measured using a full blood count. Unstimulated CD4+ lymphocytes were separated and analysed for changes in free ([Ca2+]i) and total ([Ca2+]t) calcium using flow cytometry. There were no significant differencesin leukocyte concentration between UT and TR groups. There were significant differences between TR and UT in [Ca2+]i (October B and November), and [Ca2+]t (January and March). There were also significant sequential monthly changes in both [Ca2+]i and [Ca2+]t for TR and UT groups during the study. Significant increases in [Ca2+]i and [Ca2+]t during summer (January and March) for both TR and UT groups suggest an increase in intracellular signalling during hot weather. [Ca2+]i and [Ca2+]t were significantly lower in TR lymphocytes during November and March, suggesting that endurance training during warmer months may decrease [Ca2+]i through altered intracellular signalling, possibly to maintain lymphocyte function during heat stress.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

98

Issue

3

Start Page

242

End Page

249

Number of Pages

8

eISSN

1439-6327

ISSN

1439-6319

Location

Germany

Publisher

Springer

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Griffith University; Massey University; TBA Research Institute;

Era Eligible

Yes

Journal

European journal of applied physiology.

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