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Effects of canola proteins and hydrolysates on adipogenic differentiation of C3H10T/2 mesenchymal stem cells

journal contribution
posted on 28.06.2018, 00:00 authored by AM Alashi, CL Blanchard, RJ Mailer, SO Agboola, AJ Mawson, RE Aluko, Padraig Strappe
This study assessed the ability of canola protein isolate (CPI) and enzymatic hydrolysates (CPHs) to inhibit adipogenic differentiation of C3H10T1/2 murine mesenchymal stem cells in vitro. Cell viability was maintained at concentrations of 60 μg/ml of sample. Cells treated with Alcalase hydrolysate demonstrated a higher reduction in anti-adipogenic differentiation through quantitation by oil-red O staining. qPCR analysis showed that CPI and CPH-treated cells significantly inhibited PPARγ expression, a key transcription factor involved in adipocyte differentiation, as evident in an ∼60-80% fold reduction of PPARγ mRNA. Immunofluorescence staining for PPARγ protein also showed a reduced expression in some treated cells when compared to differentiated untreated cells. The 50% inhibition concentration (IC50) of CPI, CPHs and their membrane ultrafiltration fractions on pancreatic lipase (PL) activity ranged between 0.75 and 2.5 mg/ml, (p < 0.05) for the hydrolysed and unhydrolysed samples. These findings demonstrate that CPI and CPHs contain bioactive components which can modulate in vitro adipocyte differentiation.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

185

Start Page

226

End Page

232

Number of Pages

7

eISSN

1873-7072

ISSN

0308-8146

Publisher

Elsevier, Netherlands

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

University of Manitoba, Canada

Era Eligible

Yes

Journal

Food Chemistry