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Effects of canola proteins and hydrolysates on adipogenic differentiation of C3H10T/2 mesenchymal stem cells
journal contribution
posted on 2018-06-28, 00:00 authored by AM Alashi, CL Blanchard, RJ Mailer, SO Agboola, AJ Mawson, RE Aluko, Padraig StrappeThis study assessed the ability of canola protein isolate (CPI) and enzymatic hydrolysates (CPHs) to inhibit adipogenic differentiation of C3H10T1/2 murine mesenchymal stem cells in vitro. Cell viability was maintained at concentrations of 60 μg/ml of sample. Cells treated with Alcalase hydrolysate demonstrated a higher reduction in anti-adipogenic differentiation through quantitation by oil-red O staining. qPCR analysis showed that CPI and CPH-treated cells significantly inhibited PPARγ expression, a key transcription factor involved in adipocyte differentiation, as evident in an ∼60-80% fold reduction of PPARγ mRNA. Immunofluorescence staining for PPARγ protein also showed a reduced expression in some treated cells when compared to differentiated untreated cells. The 50% inhibition concentration (IC50) of CPI, CPHs and their membrane ultrafiltration fractions on pancreatic lipase (PL) activity ranged between 0.75 and 2.5 mg/ml, (p < 0.05) for the hydrolysed and unhydrolysed samples. These findings demonstrate that CPI and CPHs contain bioactive components which can modulate in vitro adipocyte differentiation.
Funding
Category 2 - Other Public Sector Grants Category
History
Volume
185Start Page
226End Page
232Number of Pages
7eISSN
1873-7072ISSN
0308-8146Publisher
Elsevier, NetherlandsPublisher DOI
Peer Reviewed
- Yes
Open Access
- No
External Author Affiliations
University of Manitoba, CanadaEra Eligible
- Yes